Molecular Characterization and Expression Profiles of Myrosinase Gene（RsMyr2） in Radish（Raphanus sativus L.）

Myrosinase is a defense-related enzyme and is capable of hydrolyzing glucosinolates into a variety of compounds, some of which are toxic to pathogens and herbivores. Many studies revealed that a number of important vegetables or oil crops contain the myrosinase-glucosinolate system. However, the related promoter and genomic DNA sequences as well as expression profiles of myrosinase gene remain largely unexplored in radish（Raphanus sativus）. In this study, the 2 798 bp genomic DNA sequence, designated as RsMyr2, was isolated and analyzed in radish. The RsMyr2 consisting of 12 exons and 11 introns reflected the common gene structure of myrosinases. Using the genomic DNA walking approach, the 5′-flanking region upstream of RsMyr2 with length of 1 711 bp was successfully isolated. PLACE and PlantCARE analyses revealed that this upstream region could be the promoter of RsMyr2, which contained several basic cis-regulatory elements including TATA-box, CAAT-box and regulatory motifs responsive to defense and stresses. Furthermore, recombinant pET-RsMyr2 protein separated by SDS-PAGE was identified as myrosinase with mass spectrometry. Real-time PCR analysis showed differential expression profiles of RsMyr2 in leaf, stem and root at different developmental stages（e.g., higher expression in leaf at cotyledon stage and lower in flesh root at mature stage）. Additionally, the RsMyr2 gene exhibited up-regulated expression when treated with abscisic acid（ABA）, methyl jasmonate（MeJA） and hydrogen peroxide（H2O2）, whereas it was down-regulated by wounding（WO） treatment. The findings indicated that the expression of RsMyr2 gene was differentially regulated by these stress treatments. These results could provide new insight into elucidating the molecular characterization and biological function of myrosinase in radish.     

Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek’s Diseases

To investigate the dynamic change of heat shock protein 90 （Hsp90） in the genesis and development of tumor, we successfully established tumor animal model using Marek’s disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzyme-linked immunosorbent assay （ELISA） method. Our result showed that Hsp90 location in the tumor tissue was signiifcantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most signiifcantly increased with the development of tumor. This is the ifrst report to show the presence of Hsp90 in tumor tissues induced by the Marek’s disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.     

关中葡萄二黄斑叶蝉种群动态和空间分布型研究

在西北农林科技大学园艺试验站选择历年葡萄二黄斑叶蝉发生较严重的葡萄品种为调查对象,于2013年4~10月进行系统调查,并采用Taylor幂法则、Iwao回归分析法和Lloyd平均聚块性指标对葡萄二黄斑叶蝉种群聚集扩散情况进行测定分析,研究了其种群空间分布型。结果表明,葡萄二黄斑叶蝉在陕西关中地区1年发生3代,若虫1年出现3个高峰。运用3种空间分布测定指标对葡萄二黄斑叶蝉空间分布型的测定结果均一致,葡萄二黄斑叶蝉在关中地区葡萄园呈聚集分布,个体之间相互吸引,聚集强度随种群密度升高而增加,且种群密度随时序动态变化呈现出扩散-聚集-扩散-聚集的规律。     

植物组织培养在育种中的应用

随着植物组织培养技术的日趋完善,在农业中应用越来越广泛,分别从单倍体育种、多倍体育种、远缘杂交育种、人工种子、筛选突变体、转基因育种和种质资源离体保存7个方面论述植物组织培养在育种中的应用.     

水稻OsGL1-2基因反义表达载体的构建

应用聚合酶链式反应技术（PCR）扩增水稻OsGL1-2基因反义片段及基因自身的启动子,并分别克隆到pUC19克隆载体上,得到含有OsGL1-2启动子＋OsGL1-2反义片段的中间载体.对重组子进行PCR检测和酶切分析并测序,结果表明,长度分别为417和2 199bp.将OsGL1-2启动子＋OsGL1-2反义片段克隆到植物表达载体pCambia1380多克隆位点,构建了该基因的植物反义表达栽体pCamGL1-2.     

稻茬麦不同耕作方式对小麦产量及其产量构成的影响

[目的]研究稻茬麦在延期播种条件下,不同耕作方式对半冬性品种小麦产量及其构成的影响,以期找出节本、高产、稳产、增效的耕作方式。[方法]小麦品种为洛麦23。采用5种种植方式。试验期间,调查小麦群体动态、产量及其结构、子粒干物质含量。[结果]稻茬小麦不同耕作方式的基本苗、高峰苗、有效穗数都表现出差异性,早播浅旋耕(方式5)这3项指标均最高;5种不同耕作方式中,打捆和鼠道犁的耕作方式(方式2)比其他耕作方式成本高,打捆方式的经济效益最低,早播浅旋耕(方式5)的经济效益最高。[结论]早播稻茬小麦可以争取足够基本苗,提高分蘖成穗率,增加有效穗数,获得高产。在秸秆还田、提高产量的同时,降低机耕作业成本是增加单位面积效益的途径之一,因此减少灭茬碎垡的作业次数可以作为改进的方向。     

玉米芯栽培平菇技术研究——以绥化市北林区为例

针对绥化市北林区平菇的代料栽培技术,以玉米芯为主要原料,从栽培模式、栽培季节、原料准备、发酵以及出菇管理几方面阐述其栽培技术要点。     

不同含油量油菜品种LECl基因表达研究

[目的]揭示不同含油量油莱品种LECl基因的遗传多样性。[方法]测定不同含油量品种不同发育时期LECl基因的表达量及含油量。[结果]甘蓝型油菜LECl基因与含油量密切相关,其相对表达量在花期和角果期均与含油量呈显著正相关,且花期的相关系数大于角果期相关系数。[结论]该研究为选育高含油量油菜品种提供理论依据。     

大果沙棘人工林种群结构点格局分析

根据对孙吴县大河口林场大果沙棘样地的调查结果,通过采用点格局分析法对大果沙棘人工林单种群、种群内不同发育阶段大果沙棘个体的分布格局与空间关联性进行了研究.结果表明,大果沙棘人工林单种群的空间点格局在所研究的尺度呈显著集群分布;不同林龄大果沙棘个体点格局在研究尺度主要表现出集群分布,并在一定的尺度范国内表现出随机分布;在种群不同发育阶段的关联性上,邻近的发育阶段在小尺度范围内表现为负相关,表明其存在种间竞争,在大尺度范围表现出无关联性,表明其相互关系并不协调,只有其中某几个发育阶段表现出正相关的关系.依靠点格局方法分析不同尺度的格局,存在明显的优越性.